Orochem Technologies is excited to introduce our latest innovation in the purification of oligonucleotides—ZARA OPC Cartridges and Plates. These polymer-based, reversed-phase products have a pore size of approximately 300 Å, making them exceptionally stable for organic and basic samples compared to traditional C8 or C18 products.
Key Features of ZARA OPC Products:
-
Versatile Application: Ideal for general oligo purification or DMT-on oligo purification.
-
Effective Impurity Removal: Eliminates salts and traces organic impurities from crude oligonucleotides, providing pure oligonucleotides suitable for sequencing primers, PCR primers, hybridization probes, and gene constructions.
-
High Purity Output: Capable of purifying a wide range of oligos with different molecular weights, from 15 mers to 80 mers.
-
Efficient Separation: Effectively separates DMT-on oligos from other impurities.
General Protocol for Purification of Oligos:
-
Flow Rate: Regulate the flow rate of solvents through the cartridge at 1-2 drops/sec.
-
Sample Preparation: Use amino incubation solution of synthesized CPGs.
-
Conditioning: Condition the OPC product with 2 x 0.5 mL of methanol, followed by 0.5 mL of acetonitrile, then 2 x 0.5 mL 2.0 M TEAA.
-
Loading: Load the deprotection mixture (0.1 - 0.3 mL, NH4OH or AMA mixture).
-
Washing: Wash the OPC with 2 x 1 mL of ammonium hydroxide(1:20), followed by 1 mL of DI water and 0.4 mL of 2% (v/v) acetonitrile in water.
-
Elution: Elute oligonucleotide from OPC with 0.2 mL of 30% ACN/water. Collect the eluted fraction.
General Protocol for Purification of DMT-on Oligo:
-
Flow Rate: Regulate the flow rate of solvents through the cartridge at 1-2 drops/sec.
-
Sample Preparation: Use amino incubation solution of synthesized CPGs.
-
Conditioning: Condition the OPC cartridge with 2 x 0.5 mL of methanol, followed by 0.5 mL of acetonitrile, then 2 x 0.5 mL 2.0 M TEAA.
-
Loading: Load the ammonium incubation mixture (0.1 mL, NH4OH or AMA mixture).
-
Washing: Wash the OPC with 2 x 1 mL of ammonium hydroxide(1:20), followed by 1 mL of DI water.
-
Detritylation: Detritylate support-bound oligonucleotide with 2 x 0.5 mL of 2% TFA. A faint pink/orange band may be observed in the cartridge.
-
Final Wash and Elution: Wash the OPC with 2 x 0.5 mL of water and elute the oligonucleotide from OPC with 0.2 mL of 30% ACN/water. Collect the eluted fraction.
For more information and to learn more about our ZARA OPC Cartridges/Plates, please downloader our flyer or visit our website at www.orochem.com
Contact us directly:
-
Phone: 630 210 8300
-
Email: info@orochem.com