Orochem Technologies is proud to highlight our Universal Filter Plate, a cutting-edge solution designed for the efficient and reproducible clarification of cell lysates from bacterial, insect, and human sources. This versatile apparatus supports high-throughput filtration in 24-well, 48-well, and 96-well plate formats, as well as cartridges, transforming the process of cell debris and contaminant removal before the isolation of target molecules like proteins.
Key Features:
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High-throughput capability: Available in 96-well plate format and small-scale single-use depth filtration systems for larger sample volumes (up to 12 mL).
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Efficiency: Offers rapid processing, reducing the clarification time to just 5 minutes while ensuring high protein and sample volume recovery.
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Superior results: Provides outcomes comparable or superior to high-speed centrifugation, enhancing the downstream processing of particulate-free feed.
Procedure Overview:
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Perform lysate clarification at room temperature or at 4°C.
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Place the filter plate on top of a collection plate and apply up to 800 µL of cell lysate to each well.
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Use a variable-speed bench-top centrifuge with appropriate carriers, balancing the assembly before centrifuging at 1000 x g for 2 minutes to collect clarified cell lysate.
The apparatus features an open-pore structure filter to remove cells and cell debris, while a tighter pore structure filter-aid material simultaneously removes colloidal matter. This dual-action filtering system ensures high-quality clarification, making it an essential tool for researchers and laboratories.
Experimental Data:
Research conducted by the Protein Expression Laboratory at the Frederick National Laboratory underscores the effectiveness of Orochem’s Cell Lysate Filter Plate. Comparative SDS Page results demonstrated significant improvement in lysate clarification using the filtration method compared to traditional centrifugation.
To learn more about Orochem's Universal Filter Plate and its applications, please visit this link.
For further information and purchasing inquiries, contact us:
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Phone: 630 210 8300
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Email: info@orochem.com